early passage normal human female colon fibroblast ccd 18co cell line  (ATCC)

Journal: Investigational New Drugs

Article Title: Scorpio fuscus venom as a promising anticancer agent against colorectal cancer

doi: 10.1007/s10637-026-01598-z

Figure Lengend Snippet: Histopathology and immunohistochemistry (IHC) analyses. a Representative H&E-stained section from the control group showing normal intestinal architecture with preserved crypt structures (arrows). b H&E-stained section from the CDX group displaying disrupted tissue architecture, densely packed malignant epithelial cells, and invasive tumor margins (arrow). c Immunohistochemical staining for Bax in tumor tissues from the CDX + SFV group, demonstrating increased pro-apoptotic protein expression (arrows). d Immunohistochemical staining for BcL-2 in tumor tissues from the CDX group, indicating strong anti-apoptotic protein expression (arrows). Scale bars: 100 µm ( a , b ), 10 µm ( c , d )

Article Snippet: Human CRC cell lines (DLD-1, HT-29, Caco-2) and human healthy colon epithelial cell line (CCD-18Co) were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Histopathology, Immunohistochemistry, Staining, Control, Immunohistochemical staining, Expressing

Journal: Frontiers in Immunology

Article Title: From multi-omics to functional validation: the PTMRS stratifies TME and positions PDGFRB in CRC biology

doi: 10.3389/fimmu.2025.1728291

Figure Lengend Snippet: Impact of PDGFRb perturbation within tumor-associated fibroblasts on CRC cell phenotypes. (A) Western blots of t-PDGFRb and p-PDGFRb in CCD-18Co fibroblasts treated with vehicle (Veh), PDGF-BB, or PDGF-BB plus SUN. b-actin serves as a loading control. (B) Quantification of the p-PDGFRb/t-PDGFRb ratio relative to the Veh group. (C) Representative EdU staining of HCT116 cells cultured with CM from the three fibroblast conditions (Veh-CM, PDGF-BB-CM, SUN-CM). EdU (green), DAPI (blue), merged images as indicated. (D) Relative EdU labeling index in HCT116 cells. (E, F) CCK-8 assays showing relative cell viability of HCT116 (E) and DLD-1 (F) cells after 48 h exposure to Veh-CM, PDGF-BB-CM, or SUN-CM. (G) Representative Transwell images of migrated HCT116 and DLD-1 cells under the indicated CM conditions. (H, I) Quantification of migrated HCT116 (H) and DLD-1 (I) cells per 10× field. (J) Violin plots showing concentrations of CCL5, CXCL12, IL-6, IL-10, and TGF-b1 in fibroblast CM measured by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001 vs Veh group; ns, no significant difference. Abbreviations: Veh, vehicle; SUN, sunitinib; CM, conditioned medium; PDGF-BB, the BB homodimer of platelet-derived growth factor.

Article Snippet: Human CRC cell line HCT116 and colonic fibroblast cell line (CCD-18Co) were obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Western Blot, Control, Staining, Cell Culture, Labeling, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Scientific Reports

Article Title: Investigating the potential selective effects of a Kiperin multi-component magnesium supplement on colon cancer and normal cells

doi: 10.1038/s41598-025-32854-6

Figure Lengend Snippet: Cell counts of HCT-116 and CCD-18Co cell lines following treatment with 2.5 μL of magnesium. Non-treated cells group was used as a control group. The treatment led to a decrease in the number of HCT-116 cells, whereas an increase was observed in CCD-18Co cells.

Article Snippet: Two cell lines were used: CCD-18Co human colon fibroblast cells (CRL-1459) and HCT-116 human colon cancer cells (CCL-247), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: Scientific Reports

Article Title: Investigating the potential selective effects of a Kiperin multi-component magnesium supplement on colon cancer and normal cells

doi: 10.1038/s41598-025-32854-6

Figure Lengend Snippet: Scratch wound assay for evaluating the cell migration of CCD-18Co and HCT116 cell lines at 0, 6, 18 and 24 h after 2.5 μL of magnesium treatment. Non-treated cells group was used as a control group.

Article Snippet: Two cell lines were used: CCD-18Co human colon fibroblast cells (CRL-1459) and HCT-116 human colon cancer cells (CCL-247), both obtained from the American Type Culture Collection (ATCC).

Techniques: Scratch Wound Assay Assay, Migration, Control

Journal: Scientific Reports

Article Title: Investigating the potential selective effects of a Kiperin multi-component magnesium supplement on colon cancer and normal cells

doi: 10.1038/s41598-025-32854-6

Figure Lengend Snippet: Gene expression in CCD-18Co cells with and without 2.5 μL magnesium supplement. VDR and TNFα gene expression levels were significantly altered. Non-treated cells group was used as a control group.

Article Snippet: Two cell lines were used: CCD-18Co human colon fibroblast cells (CRL-1459) and HCT-116 human colon cancer cells (CCL-247), both obtained from the American Type Culture Collection (ATCC).

Techniques: Gene Expression, Control